Device

Part:BBa_K598011:Design

Designed by: Yangyang ZHAO, Zhenrun ZHANG, Yan GONG   Group: iGEM11_pku_riboclamp   (2011-09-27)


pBAD+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 931


Design Notes

Use it by stantard assembly or by sites mutation


Source

E.coli

References